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Release 5.4.0 (Yellow Frog)#970

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jfy133 merged 91 commits intomainfrom
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Feb 2, 2026
Merged

Release 5.4.0 (Yellow Frog)#970
jfy133 merged 91 commits intomainfrom
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@jfy133 jfy133 commented Jan 26, 2026

PR checklist

  • This comment contains a description of changes (with reason).
  • If you've fixed a bug or added code that should be tested, add tests!
  • If you've added a new tool - have you followed the pipeline conventions in the contribution docs
  • If necessary, also make a PR on the nf-core/mag branch on the nf-core/test-datasets repository.
  • Make sure your code lints (nf-core pipelines lint).
  • Ensure the test suite passes (nextflow run . -profile test,docker --outdir <OUTDIR>).
  • Check for unexpected warnings in debug mode (nextflow run . -profile debug,test,docker --outdir <OUTDIR>).
  • Usage Documentation in docs/usage.md is updated.
  • Output Documentation in docs/output.md is updated.
  • CHANGELOG.md is updated.
  • README.md is updated (including new tool citations and authors/contributors).

PetcuBogdan and others added 30 commits October 23, 2025 10:13
Co-authored-by: Daniel Straub <[email protected]>
@jfy133 jfy133 marked this pull request as ready for review January 26, 2026 13:20
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github-actions Bot commented Jan 26, 2026

nf-core pipelines lint overall result: Passed ✅ ⚠️

Posted for pipeline commit 223ef48

+| ✅ 382 tests passed       |+
#| ❔   1 tests were ignored |#
!| ❗   7 tests had warnings |!
Details

❗ Test warnings:

  • nextflow_config - Config manifest.version should end in dev: 5.4.0
  • pipeline_todos - TODO string in main.nf: Remove this line if you don't need a FASTA file [TODO: try and test using for --host_fasta and --host_genome]
  • pipeline_todos - TODO string in methods_description_template.yml: #Update the HTML below to your preferred methods description, e.g. add publication citation for this pipeline
  • pipeline_todos - TODO string in main.nf: Optionally add in-text citation tools to this list.
  • pipeline_todos - TODO string in main.nf: Optionally add bibliographic entries to this list.
  • pipeline_todos - TODO string in main.nf: Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled!
  • pipeline_todos - TODO string in nextflow.config: Specify any additional parameters here

❔ Tests ignored:

  • files_unchanged - File ignored due to lint config: .github/PULL_REQUEST_TEMPLATE.md

✅ Tests passed:

Run details

  • nf-core/tools version 3.5.1
  • Run at 2026-02-02 11:16:59

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@ggabernet ggabernet left a comment

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Looks good to me, just a couple of tests seem not to be passing with singularity

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@charles-plessy charles-plessy left a comment

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The changes look good to me and @takiifujita-ui. We did not proofread the python code in details.

Comment thread subworkflows/local/binning_pydamage/meta.yml
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Warning

Newer version of the nf-core template is available.

Your pipeline is using an old version of the nf-core template: 3.5.1.
Please update your pipeline to the latest version.

For more documentation on how to update your pipeline, please see the nf-core documentation and Synchronisation documentation.

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jfy133 commented Feb 2, 2026

Final issue is due to

  [f8/066e9b] Submitted process > NFCORE_MAG:MAG:ASSEMBLY:POOL_SHORT_READS (group-0)
  [cd/35613a] Submitted process > NFCORE_MAG:MAG:ASSEMBLY:SHORTREAD_ASSEMBLY:MEGAHIT (group-0)
  ERROR ~ No such file or directory: https://zenodo.org/records/14897628/files/checkm2_database.tar.gz
  

Which I am replicating locally when running with conda and nf-test. This is is nothing we can do about currently as per https://blog.zenodo.org/2026/01/28/2026-01-28-improvements-and-support-expectations/

@jfy133 jfy133 merged commit b52fa53 into main Feb 2, 2026
150 of 157 checks passed
cavenel added a commit to nf-core/spatialvi that referenced this pull request Mar 20, 2026
See https://nfcore.slack.com/archives/C04QR0T3G3H/p1770036127541799:
We pushed a patch-release for nf-core tools (3.5.2) without any template changes. Unfortunately, this still results in the outdated comment being added to your PRs, e.g., nf-core/mag#970 (comment). Sorry, for that.
Either ignore them or just update the nf-core version in .nf-core.yml to 3.5.2
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